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Title

Isolation and Characterization of Campylobacter jejuni TGH 9011 and

Capsular Polysaccharide Biosynthetic Locus

Only the First Letter of each word should be capitalized. Only scientific names should be in italics. Do not bold any part of the title. Do not add a period at the end of the title

Author Block

Cory N. Cates, Margaret Kanipes.
North Carolina A&T State University, Greensboro, NC, USA.

You must have at least 2 authors in the author block. The first author must be the presenting author.

Abstract

Campylobacter jejuni is one of the principal causes of bacterial illness throughout the world. This organism possesses a unique set of genes responsible for the biosynthesis of capsular polysaccharides (CPSs), which are cell surface structures found on a great number of bacterial species. CPSs are important in the survival of bacteria and play a major role in pathogenesis. One of the goals of our research is to characterize the CPS biosynthetic locus in Campylobacter strains and understand its role in virulence. We hypothesize that the C. jejuni TGH9011 CPS locus would show significant gene conservation as well as new genetic content compared to the LOS loci from known C. jejuni strains. In this study, the CPS region of C. jejuni strain CG 8486 was used as a template to generate primers in order to isolate and characterize the C. jejuni TGH9011 CPS region, via PCR. Five DNA fragments (A-E) which encompass the 22 kb CPS locus of C. jejuni TGH9011 were isolated by PCR. Thus far, DNA fragments A-E have been sequenced and analyzed. Analysis of the data from these regions of the C. jejuni TGH9011 CPS locus show significant homology to the CPS biosynthetic loci from other Campylobacter strains. These studies on the CPS locus could play a major role in the development of a classification method for this organism.

Limited to 2,500 characters, not including spaces. Do not include the title or author block to this section. Citations are not allowed. In order to be accepted, your abstract must contain a hypothesis / statement of problem, experimental methods/methodology used, results even if preliminary, and a conclusion.

Scientific Discipline

1. Biochemical Sciences: a. Biochemistry

Only one of the ten available disciplines can be selected from the drop-down box. This will be used to assign your abstract to the appropriate reviewers and on-site judges.